Catalogue

Background
Vascular endothelial growth factor and type I collagen inducible protein (VCIP), also known as phosphatidic acid phosphatase 2b (PAP2b), was originally identified in a functional assay of angiogenesis. VCIP/PAP2b exhibits an ArgÐGlyÐAsp (RGD) cell adhesion sequence. Immunoprecipitation and fluorescence-activated cell sorting analyses demonstrated that VCIP-RGD is exposed to the outside of the cell surface. Retroviral transduction of VCIP can induce cell aggregation and cellÐ cell interactions, modestly increase p120 catenin expression and promote activation of the Fak, Akt and GSK3§ protein kinases. Expression of VCIP promoted adhesion, spreading and tyrosine phosphorylation of Fak, Shc, Cas and paxillin in endothelial cells. PAP2b/VCIP is expressed in close proximity to vascular endothelial growth factor, von Willebrand factor and {alpha}v§3 integrin in tumor vasculatures.

Synonyms: PAP2B

Source

Immunogen: Synthetic peptide derived from the phosphatidic acid phosphatase 2B protein

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen Immunoaffiinity Purification

Concentration: Lot specific, see vial

Applications
Antibody can be used for Western blotting (see vial for dilution) and EIA. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Human brain lysate (Cat. No. X1633C)

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
[1] Kai M., Wada I., Imai S.-I., Sakane F., Kanoh H.; Cloning and characterization of two human isozymes of Mg2+-independent phosphatidic acid phosphatase.; J. Biol. Chem. 272:24572-24578(1997). [2] Roberts R., Sciorra V.A., Morris A.J.; Human type 2 phosphatidic acid phosphohydrolases. Substrate specificity of the type 2a, 2b, and 2c enzymes and cell surface activity of the 2a isoform.; J. Biol. Chem. 273:22059-22067(1998). [3] Humtsoe J.O., Feng S., Thakker G.D., Yang J., Hong J., Wary K.K.; Regulation of cell-cell interactions by phosphatidic acid phosphatase 2b/VCIP.; EMBO J. 22:1539-1554(2003). [4] Leung D.W., Tompkins C.K.; Molecular cloning of and expression of an isoform of human phosphatidic acid phosphatase cDNA.; Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases. [5] Yu W., Andersson B., Worley K.C., Muzny D.M., Ding Y., Liu W., Ricafrente J.Y., Wentland M.A., Lennon G., Gibbs R.A.; Large-scale concatenation cDNA sequencing.; Genome Res. 7:353-358(1997). [6] Strausberg R.L., et al Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002). [7] Burnett C., Makridou P., Hewlett L., Howard K.; Lipid phosphate phosphatases dimerise, but this interaction is not required for in vivo activity.; BMC Biochem. 5:2-2(2004).

Protein Reference(s)

Database Name: UniProt

Accession Number: O14495

Species Accession: Human

Safety Datasheet(s) for this product: