Catalogue

Background
The p73 exhibits sequence homology to the p53's transcriptional activation, DNA binding, and oligomerization domains. However, adenovirus E1B 55K, simian virus 40 T, and human papillomavirus E6 do not physically interact with p73 indicating that the functions of these two proteins may differ under physiological conditions. p73 is expressed in either a full-length form ( ) (MW ~80kDa) or a shorter ( ) (MW ~70kDa) mRNA variant, with exon 13 spliced out.

Source

Immunogen: GST-fused recombinant protein derived from the C terminal of the p73 protein.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: COS monkey kidney cells and IM9 human myeloma cells

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Zeng, X., et al. 'p300 does not require its acetylase activity to stimulate p73 function.' J. Biol. Chem. 2001: 276, 48-52. 2. Dobbelstein M, Roth J: The large T antigen of simian virus 40 binds and inactivates p53 but not p73. J Gen Virol 1998;79:3079-3083. 3. Higashino F, et al: Adenovirus e4orf6 oncoprotein modulates the function of the p53-related protein, p73. Proc Natl Acad Sci U S A 1998 Dec 22;95(26):15683-15687. 4. Lo Cunsolo C, et al: Refined chromosomal localization of the putative tumor suppressor gene TP73. Cytogenet Cell Genet 1998;82(3-4):199-201. 5. Kovalev S, et al: Expression level, allelic origin, and mutation analysis of the p73 gene in neuroblastoma tumors and cell lines. Cell Growth Differ 1998 Nov;9(11):897-903. 6. Zhu J, et al: The potential tumor suppressor p73 differentially regulates cellular p53 target genes. Cancer Res 1998 Nov 15;58(22):5061-5065. 7. De Laurenzi V, et al: Two new p73 splice variants, gamma and delta, with different transcriptional activity. J Exp Med 1998 Nov 2;188(9):1763-1768. 8. Nimura Y, et al: p73, a gene related to p53, is not mutated in esophageal carcinomas. Int J Cancer 1998 Nov 9;78(4):437-440. 9. Marin MC et. al. Viral oncoproteins discriminate between p53 and the p53 homolog p73. Molecular and Cellular Biology, 1998;18(11):6316-6324.

Protein Reference(s)

Database Name: SwissProt

Accession Number: O15350

Species Accession: Human

Safety Datasheet(s) for this product: