Catalogue

Background
Broad-specificity phosphohydrolase that dephosphorylates exogenous bioactive glycerolipids and sphingolipids. It catalyzes the conversion of phosphatidic acid (PA) to diacylglycerol (DG), and is a pivotal regulator of lysophosphatidic acid (LPA) signaling in the cardiovascular system. It is a major enzyme responsible of dephosphorylating LPA in platelets, which terminates signaling actions of LPA. It may control circulating, and possibly also regulate localized, LPA levels resulting from platelet activation. It has little activity towards ceramide-1-phosphate (C-1-P) and sphingosine-1-phosphate (S-1-P). The relative catalytic efficiency is LPA > PA > S-1-P > C-1-P. Its down-regulation may contribute to the development of colon adenocarcinoma.

Synonyms: EC 3.1.3.4, Lipid Phosphate Phosphatase 1, Phosphatidic acid phosphatase 2a

Source

Immunogen: Synthetic peptide derived from the LPP1 protein

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Antigen affinity purified

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (5-10 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Roberts, RZ, Morris, AJ. 'Role of phosphatidic acid phosphatase 2a in uptake of extracellular lipid phosphate mediators.' Biochim Biophys Acta, 2000, 1487, 33-49 2. Roberts, RZ, Sciorra, VA, Morris, AJ. 'Human type 2 phosphatidic acid phosphohydrolases. Substrate specificity of the type 2a, 2b and 2c enzymes and cell surface activity of the 2a isoform.' J Biol Chem, 1998, 273, 22059-22067

Protein Reference(s)

Database Name: UniProt

Accession Number: O14494(Human)

Species Accession: Human

Safety Datasheet(s) for this product: