Catalogue

Background
Ceramide Glycosyltransferase may serve as a flippase as well as a glucosyltransferase that transfers glucose to ceramide. It Catalzes; UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine. Ceramide Glycosyltransferase is the first step in the Glycosphingolipid synthesis; first glycosylation step. Glucosylceramide synthase (GlcT) and lactosylceramide synthase (GalT) are key enzymes for the synthesis of major glycosphingolipids of vertebrates.

Synonyms: Glucosylceramide synthase, GCS, UDP glucose N-acylsphingosine D glucosyltransferase, GLCT-1, UDP glucose ceramide glucosyltransferase

Source
Rabbits were immunized with a synthetic peptide derived from human ceramide glycosyltransferase protein.

Product
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Product Form: Unconjugated

Purification Method: Ammonium Sulfate Precipitation

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (5-10µg/ml) and ELISA. Other applications not yet tested. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Found in all tissues examined.

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Expression cloning of a cDNA for human ceramide glucosyltransferase that catalyzes the first glycosylation step of glycosphingolipid synthesis.; Ichikawa S., Sakiyama H., Suzuki G., Hidari K.I.-P., Hirabayashi Y.; Proc. Natl. Acad. Sci. U.S.A. 93:4638-4643(1996). 2. van Vlerken, L.E., et al. ‘Modulation of Intracellular Ceramide Using Polymeric Nanoparticles to Overcome Multidrug Resistance in Cancer.’ Cancer Res., 67, 4843-4850, (2007).

Protein Reference(s)

Database Name: UniProt

Accession Number: Q16739

Species Accession: Human

Safety Datasheet(s) for this product: