Catalogue

Background
Negative control for immunofluorescence staining with FITC conjugated mouse monoclonal antibodies. Assessment of non-specific binding of mouse monoclonal antibodies to human cell surface antigens.

Product
Purified mouse IgG2a conjugated to FITC, as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Consult the appropriate fact sheet to determine the amount of antibodies to be used as a control for PBMC or Whole Blood. PBMC: Add10 μl of MAB/10^6 PBMC in 100 μl PBS. Mix gently and incubate for 15 minutes at 2° to 8°C. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 μl of MAB/100 μl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

Working Concentration: Titered for flow cytometry

Storage
Product should be stored at 4-8°C. DO NOT FREEZE. Store protected from light. Reagent is stable for the period shown on the vial when stored properly.

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

Warranty
Reagent is stable for the period shown on the vial when stored properly.

Protein Reference(s)

Accession Number: Swiss Prot P01863

Safety Datasheet(s) for this product: