Catalogue
Mouse anti Human p53 (a.a. 371-380)
Catalog number: X1493M$319.00
Add To CartClone | HR231 |
Isotype | IgG2b |
Product Type |
Monoclonal Antibody |
Units | 100 µg |
Host | Mouse |
Species Reactivity |
Human Monkey Mouse Rat |
Application |
ELISA Immunohistochemistry Immunoprecipitation Western Blotting |
Background
p53 is a 53 kDa transcription factor that can inhibit cell cycle progression or induce apoptosis in response to stress or DNA damage. Disruption of the p53 signalling pathway through various mechanisms is the most common alteration in human cancer occuring in over half of all tumors. The p53 protein is short lived and expressed at low levels in normal cells but accumulates and/or is activated in cells that have undergone genotoxic damage or oncogene activation. Many tumor derived and transformed cell lines express elevated levels of mutant p53 protein. Other genes also implicated in the downstream effects as a result of p53 activation are: p21WAF1, GADD45, 14-3-3, bax, Fas/APO1, KILLER/ DR5, Tsp1, IGF-BP3 and others.
Source
Hybridoma produced by the fusion of splenocytes from immunized with full length human p53 protein and mouse myeloma cells.
Product
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Applications
This antibody can be used for Western blotting (1-5 µg/ml), immunoprecipitation, ELISA and immunohistochemistry. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Breast or colon carcinoma cells
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1. Zeng, X., et al. UV but not gamma irradiation accelerates p53-induced apoptosis of teratocarcinoma cells by repressing MDM2 transcription. Cancer Res. 2000, 60, 6184-6188
2. Hussain, S.P., et al. p53 tumor suppressor gene: at the crossroads of molecular carcinogenesis, molecular epidemiology and human risk assessment. Ann. N.Y. Acad. Sci. 2000, 919, 79-85
3. Hellin, A.C., et al. Roles of Nuclear Factor-kappaB, p53 and p21/WAF1 in Daunomycin-induced cell cycle arrest and apoptosis. J. Pharmacol. Exp. Ther. 2000, 295, 870-878
Protein Reference(s)
Database Name: SwissProt
Accession Number: Q9BRQ8
Species Accession: Human
Safety Datasheet(s) for this product:
EA_Sodium Azide |
" Western blot analysis using p53 cln HR231 antibody at 1 µg/ml on native H1299 cells (1) and H1299 cells transfected with human p53. Also shown is a graphic representation of the epitope location." |
" Western blot analysis using p53 cln HR231 antibody at 1 µg/ml on native H1299 cells (1) and H1299 cells transfected with human p53. Also shown is a graphic representation of the epitope location."