Catalogue

Background
p21, also known as wild-type p53-activated factor (WAF-1) or Cdk-interacting protein (CIP1), expression is predominantly induced by p53. This expression is usually in response to DNA damage caused by cytotoxic agents. The expression of p21 interrupts the cell cycle and prevents the replication of the cell containing genomic errors. p21 inhibits Cdk2, Cdk3, Cdk4 and Cdk6 to stop the cell cycle. Contained in the human p21 is a highly conserved cystine-rich region (amino acids 21-60) with a potential zinc finger domain as well as a second conserved region (amino acids 130-164) which contains several putative nuclear localization signals. p21 can also interact with proliferating cell nuclear antigen (PCNA), Gadd45, Cdk2 and cyclins which suggests it may coordinate DNA repair and replication in damaged cells.

Source

Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with human p21 protein and mouse myeloma SP2 cells.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Detects p21 protein by Western blot at 1 to 10 µg/ml. Detects a 21 kDa protein in MCF7 cells. Can be used with either frozen or paraffin embedded tissue sections. Optimal concentration should be evaluated by serial dilutions. Other Applications: ELISA

Functional Analysis: Western Blotting

Positive Control: MCF7 cell lysate

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Hellin, A.C., et al. Roles of Nuclear Factor-kappaB, p53 and p21/WAF1 in Daunomycin-induced cell cycle arrest and apoptosis. J. Pharmacol. Exp. Ther. 2000, 295, 870-878 2. El-Deiry, W.S., et al. WAF1, a potential mediator of p53 tumor suppression. Cell 1993, 75, 817-825 3. Xiong, Y., et al. p21 is a universal inhibitor of cyclin kinases. Nature (Lond) 1993, 366, 701-704 4. Zhang, H., et al. Proliferating cell nuclear antigen and p21 are components of multiple cell cycle kinase complexes. Mol. Biol. Cell 1993, 4, 897-906 5. Kovalic, J., et al. Int. J. Oncol. 1996, 9 (suppl.), 835 6. Shen, J., et al. ‘Identification and Validation of Differences in Protein Levels in Normal, Premalignant, and Malignant Lung Cells and Tissues Using High-Throughput Western Array and Immunohistochemistry.’ Cancer Res., 66, 11194-11206 (2006).

Protein Reference(s)

Database Name: SwissProt

Accession Number: P38936

Species Accession: Human

Safety Datasheet(s) for this product: