Catalogue

Background
Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo- orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl- sphingomyelin was the most effective, followed by palmitoyl- sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group (By similarity).

Synonyms: HNK1, GLCATP, NK-1, HNK-1, LEU7, NK1, 151290, B3GAT1, Q9P2W7, GlcUAT-P, CD57, GlcUATP, GlcAT-P, 27087, NK 1, HNK 1, GlcAT P, GlcUAT P

Source

Immunogen: Hybridoma produced from mice immunized with human peripheral blood mononuclear cells.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (1-10 µg/ml), Immunoprecipitation (1-2 µg per 100-500 µg of total protein), Immunofluorescence (2-20 µg/ml), Immunohistochemistry (2-20 µg/ml). Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Human lymph node, Tonsil

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Product Stability: See expiration date on vial

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
Abo, T., et al. J. Immuno., 127, 1024 (1984)

Protein Reference(s)

Database Name: SwissProt

Accession Number: Q9P2W7

Species Accession: Human

Safety Datasheet(s) for this product: