Catalogue
Mouse anti Human bcl-xl
Catalog number: A110M$319.00
Add To CartClone | 2H12 |
Isotype | IgG2a |
Product Type |
Monoclonal Antibody |
Units | 100 µg |
Host | Mouse |
Species Reactivity |
Human Mouse Rat |
Application |
Western Blotting |
Background
Overexpression of bcl-xl to other bcl-2 members is believed to promote cell survival. The ratio of bcl-xl to other family members is believed to modulate the apoptotic process.
Synonyms: bcl-xl, mouse anti human bcl xl, bclxl
Source
Mice were immunized with a synthetic peptide corresponding to amino acids 3 to 14 of the human bcl-xl sequence.
Immunogen: A synthetic peptide corresponding to amino acids 3 to 14 of the human bcl-xl sequence
Product
Provided as solution in phosphate buffered saline.
Product Form: Unconjugated
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Applications
Application: Western Blot at 1-5 ug/ml.
Functional Analysis: Western Blotting
Positive Control: Human, mouse and rat thymocytes
Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References
1) L.H. Boise, et. al. (1993) Cell, 74:597-608.
2) Y.T. Hsu et. al. (1997) J. Biol. Chem., 272(21): 13829-13834
3) Y. T. Hsu et. al (1997) Proc. Natl. Acad. Sci. USA, 94: 3668-3672
Protein Reference(s)
Database Name: UniProt
Accession Number: Q07817
Western blot analysis of thymocytes from human, mouse and rat. Cell lysates from approximately 106 cells were loaded into each lane of a 12% SDS-PAGE gel and blotted onto PVDF membranes. Exalpha’s anti-Bcl-XL antibody was used at 1 µg/ml - detection was by chemiluminescence detection using an anti-mouse secondary antibody.