Catalogue

Source
Chickens were immunized with FLAG Epitope (DYKDDDDK) conjugated to KLH. After multiple immunizations, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support.

Product

Product Form: Affinity purified

Formulation: Phosphate buffered saline, pH 7.2 with 0.09% sodium azide

Concentration: See vial for concentration.

Applications
Antibody was analyzed using amino-terminal Met-Flag-BAP, amino- terminal FLAG-BAP and carboxy-terminal FLAG-BAP fusion proteins and Invitrogen Positope R900-40. ELISA: We suggest a 1:5,000-1:10,000 dilution. WESTERN BLOTS: We suggest a 1:2,000 to 1:10,000 dilution. For immunohistochemistry, we suggest a 1:500 dilution (see image). Please note that the investigator must determine the appropriate dilution for your particular application.

Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles.

Shipping Conditions: Ship at ambient temperature.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.

References
1. Murshid, A., Srivastava, A., Kumar, R and J. F. Presley, 2006, Characterization of the localization and function of NECAP 1 in neurons. Journal of Neurochemistry 98 (6): 1746–1762. 2. Martin EA, Muralidhar S, Wang Z, Cervantes DC, Basu R, Taylor MR, Hunter J, Cutforth T, Wilke SA, Ghosh A, Williams ME. The intellectual disability gene Kirrel3 regulates target-specific mossy fiber synapse development in the hippocampus Elife. 2015 Nov 17;4. pii: e09395. doi: 10.7554/eLife.09395

Safety Datasheet(s) for this product: